Acetyl-CoA Carboxylase (ACC) Activity Assay Kit

$ 362⁹⁵
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Acetyl-CoA Carboxylase (ACC) Activity Assay Kit

SKU #	E-BC-K508-M
Detection Instrument	Microplate reader (650-670 nm, optimum wavelength: 660 nm)
Detection method	Colorimetric method

Product Details

Properties

Synonyms	ACC
Sample type	Animal, plant tissue
Sensitivity	6.78 U/L
Detection range	6.78-138 U/L
Detection Method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 8% \| Average intra-assay CV: 4%
Other instruments required	Vortex mixer, Incubator, Centrifuge, 100°C water bath
Other reagents required	Double distilled water, Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Dilution of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Potato tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Detection Principle

ACC catalyzes acetyl-coA, NaHCO3 and ATP to produce inorganic phosphorus, the activity of ACC can be calculated by measuring the change of inorganic phosphorus content.

Kit Components & Storage

Item	Component	Size (96 T)	Storage
Reagent 1	Buffer Solution	30 mL × 1 vial	-20°C, 12 months
Reagent 2	Substrate A	Powder × 2 vials	-20°C, 12 months shading light
Reagent 3	Negative Control Solution	3.5 mL × 1 vial	-20°C, 12 months
Reagent 4	Substrate B	3.5 mL × 1 vial	-20°C, 12 months shading light
Reagent 5	Chromogenic Agent A	Powder × 2 vials	-20°C, 12 months shading light
Reagent 6	Chromogenic Agent B	Powder × 2 vials	-20°C, 12 months shading light
Reagent 7	Acid Solution	1.8 mL × 1 vial	-20°C, 12 months
Reagent 8	500 μmol/L Standard Solution	6 mL × 1 vial	-20°C, 12 months
	Microplate	96 wells	No requirement
	Plate Sealer	2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	12.80	56.50	103.00
%CV	4.2	4.0	3.8

Inter-assay Precision

Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	12.80	56.50	103.00
%CV	7.4	8.2	8.4

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.

	Standard 1	Standard 2	Standard 3
Expected Conc. (μmol/L)	126	265	438
Observed Conc. (μmol/L)	127.3	251.8	429.2
Recovery rate (%)	101	95	98

Sensitivity

The analytical sensitivity of the assay is 6.78 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (μmol/L)	0	100	150	200	300	400	450	500
OD value	0.040	0.181	0.243	0.315	0.452	0.596	0.670	0.733
OD value	0.040	0.172	0.242	0.312	0.458	0.599	0.671	0.744
Average OD	0.040	0.177	0.243	0.314	0.455	0.598	0.671	0.739
Absoluted OD	0.000	0.137	0.203	0.274	0.415	0.558	0.631	0.699