TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
Introduction
Elabscience® TUNEL In Situ Apoptosis Kit (HRP-DAB Method) has high sensitivity and easy operation.
This kit is suitable for in situ apoptosis detection of tissue samples (paraffin sections, frozen sections) and cell samples (cell smears, slides), and the detection results can be observed by optical microscope.
Product Details
Properties
| Application | DNA Fragmentation |
| Detection method | HRP-DAB |
| Sample type | Paraffin section, frozen section, cell slide |
| Assay time | 3 hours |
| Detection instrument | Light microscope |
| Other reagents required | 4% Paraformaldehyde(E-IR-R113), 0.2% Triton X-100(E-IR-R122), 3% H2O2(E-IR-R115), Xylene, Ethanol, DNase I，PBS(E-IR-R187), ddH2O, Hematoxylin(E-IR-R120), Neutral gum(E-IR-R118) |
| Storage | -20°C, shading light. Refer to the Manual. |
| Shipping | Ice bag |
| Expiration date | 12 months |
Detection Principle
When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with biotinlabeled dUTP, horseradish peroxidase (HRP)-labeled Streptavidin (Streptavidin-HRP) can be combined with biotin. So apoptotic cells can be observed by DAB reaction with optical microscope.
Storage
Store at -20°C, and the shelf life is one year. Streptavidin-HRP and DAB Concentrate (20×) should be stored in the dark.
Notes
- This kit is for research use only.
- Please take safety precautions and follow the procedures of laboratory reagent operation.
- The washing operation should be sufficient, otherwise it will affect the enzyme activity (such as DNase I and TdT Enzyme) subsequent experimental operations. After washing the slides with PBS, please carefully blot the liquid around the sample areas with absorbent paper.
- Keep the sample moist during the experiment to prevent the failure of the experiment caused by dry slides.
- Avoid repeated freezing and thawing of the Labeling Solution and TdT enzyme. Stirring by vortex is not recommended.
- The conditions recommended in this manual are universal. Users can optimize the sample processing time, reagent concentration and other conditions according to different sample types and pre-experiment results, and select the most suitable experimental conditions.