DNA and RNA amplification methods have taken significant importance in today’s biotechnology to progress the field of research, diagnostic, and therapeutic intervention. The capacity to identify, measure, and compare nucleic acids is crucial earlier than many discoveries. At the tip of this technological spear is Bioneer, a biotech company in a league of its own – distributed by MSE Supplies. With a rich history and a commitment to innovation, Bioneer offers an extensive range of DNA/RNA amplification products that cater to a variety of scientific needs.

The Importance of DNA/RNA Amplification

DNA and RNA amplification is important in a variety of applications – from diagnosis of the presence of bacterial or viral infectious agents to analysis of differential gene expression. This makes it possible for researchers to go deep into the analysis even in conditions where the sample amount is very small by increasing the quantity of nucleic acids. It may be used for analyzing body fluids in a crime scene, for diagnosing a disease, or for medical research that aims to produce a new, effective treatment such as gene therapy.

Overview of DNA/RNA Amplification Methods

PCR or polymerase chain reaction has been among the most used over the years from all the DNA/RNA amplifications. PCR is known by the scientific world due to its credibility and can be applied in several fields. However, even in the extent of flexibility when it comes to PCR, some instance needs a special form and choosing another method is the best decision. For example, for gene expression and virus detection, Reverse Transcription PCR (RT-PCR) is employed. The choice of the method of amplification is defined by the sphere of application, the type of material taken for analysis, and the degree of sensitivity necessary.

Below is an overview of the key amplification methods available:

DNA/RNA Amplification: Applications and Impact

DNA/RNA amplification technologies have been central tool in enhancing of several areas of science and medicine. For example, PCR is still considered to be one of the most effective methods in the diagnostics of infectious diseases because it allows pinpointing the pathogen on a molecular level. qPCR and dPCR are essential techniques employed in the evaluation of gene expression profiles and detection of low-prevalence genetic mutations essential in cancer care and the identification of receptor targets for therapy.

In addition, due to its flexibility of usage, RT-PCR has become a key technique in virology with the identification and characterization of RNA viruses including SARS-CoV-2, the virus that caused the coronavirus disease 19 (COVID-19). Through the process of converting RNA to cDNA, RT-PCR helps detect gene activity and virus replication, crucial factors for disease prognosis and therapy outcomes.

When such conventional methodologies are not effective enough for high-throughput processes like gene delivery and vaccine manufacturing; that is why new and effective methods like RCA are prospective. These methods are developed as an answer to increasing demands for higher sensitivity, selectivity and productivity in the framework of modern biotechnology.

Bioneer's Commitment to Innovation and Quality

Bioneer has been at the forefront of the biotechnology and molecular biology industry. Being the first biotechnology company in Korea, Bioneer has continued to elevate the level of molecular biology through pioneering products/services and its sophisticated technologies that redefined conventional benchmarks. Ever since the early development of oligonucleotide synthesis or the more recent and progressive perfection of automated nucleic acid extraction systems, Bioneer has been a key player in defining tomorrow’s life sciences.

Bioneer prides itself in its capability in molecular biology, DNA and RNA synthesis, and process development to provide diagnostic and therapeutic products to as many people as possible. Operating both in research and clinical practice, Bioneer’s experience remains dedicated to providing solutions that effectively change people’s lives.

Bioneer's Comprehensive Range of DNA/RNA Amplification Products

Bioneer offers an extensive range of DNA/RNA amplification products designed to meet the diverse needs of researchers in the industry:

1. DNA Amplification:
• Standard PCR: Reliable amplification for a wide range of DNA sequences.
• High-Sensitivity PCR: Designed to amplify low-copy-number DNA, ensuring accurate detection.
• Long & High-Fidelity PCR: Ideal for amplifying long DNA fragments with high accuracy.
• High-Fidelity PCR: Provides enhanced accuracy for amplifying critical DNA sequences.
• Multiplex PCR: Enables the simultaneous amplification of multiple targets in a single reaction.
2. RNA Amplification (RT & RT-PCR):
• RT & RT-PCR: Efficiently convert RNA to DNA and amplify it, crucial for gene expression studies.
• High-Sensitivity RT & RT-PCR: Sensitive detection of low-abundance RNA targets.
• Long RNA RT: Facilitates the reverse transcription of long RNA sequences.
• Multiplex RT-PCR: Allows for the amplification of multiple RNA targets simultaneously.
• Dual-HotStart RT PCR: Combines HotStart technology with RT-PCR for increased specificity and reduced non-specific amplification.
3. Real-Time Gene Amplification:
• qPCR: Quantitative PCR for precise measurement of DNA quantities.
• RT-qPCR: Combines reverse transcription with qPCR for quantifying RNA levels.
4. Ligation:
• AccuPower® DNA Ligation PreMix: Streamlines the DNA ligation process with pre-mixed reagents.

In addition to these core amplification products, Bioneer also provides a comprehensive list of products & services to support your research needs:

• DNA Ladders and Markers: High-quality tools for molecular weight estimation in gel electrophoresis.
• DNA and RNA Purification Products: Efficient purification kits to ensure the integrity of nucleic acids.
• Oligonucleotide Synthesis Products: Custom and pre-made oligos for various applications, delivered with precision.
• PCR Master Mixes: Convenient, pre-mixed solutions to simplify your PCR setup.
• Phosphoramidites for DNA/RNA Synthesis: Essential building blocks for high-fidelity DNA/RNA synthesis.
• ExiPrep: Automated nucleic acid purification systems for consistent and reliable sample preparation.
• Gene Synthesis Services: Custom gene synthesis to accelerate your research projects.
• ExiSpin: A combination vortex/centrifuge designed to optimize your workflow.
• Enzymes: High-quality DNA polymerases, ligases and reverse transcriptases to enhance your amplification reactions.

MSE Supplies: Your Partner in Biotechnology

Since MSE Supplies has been a certified distributor of Bioneer products, we are pleased to announce that we are offering the following innovative solutions to the scientific world. For instance, if you are involved in academic, forensic, clinical or pharmaceutical research, MSE Supplies is here to ensure that you get the best quality products and advice.

With one another, Bioneer and MSE Supplies complement the efforts in their work for the modern development of the biotechnology industry and offer the latest biotechnology tools and reagents instrumental in helping scientists make discoveries. Contact us today!