Nest Cell Culture Inserts

Cell culture inserts are designed to be inserted into their respective culture plates, based on well dimensions. These inserts can be slotted in multiple positions, allowing you to adjust well growth surface height, as well as the resulting media volume required for such a height.

This customizability allows you to optimize for gas exchange, oxygen tension, pH, and growth factor availability. Different insert pore sizes are available depending on application. Navigate to the associated guide using the button below.

Material:

Multiple well plate or dish is made from Polystyrene (PS)

Applications:

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

Product details:

Innovative hanging design facilitates pipetting.

·Supplied in multiple well plates: 6-well, 12-well, 24well.

·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds

·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.

·Compatible with most solvents used for fixing and dyeing.

Attention：

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;

•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;

•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines for use：

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.

• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.

• Check the volume of the culture medium regularly and add fresh medium as needed.

• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.

• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.

Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Packing specification	Inserts/plate	Insert Diameter(mm)	Volume of Each Well (mL)	Inner Volume of Insert (mL)	Membrane Growth Area of Insert (cm²)
6	6 inserts/6 well	24.0	2.6	1.5	4.67
12	12 inserts/6 well	12.0	1.5	0.5	1.12
24	12 inserts/6 well	6.5	0.6	0.1	0.33
100mm dish	1 insert/6 well	75.0	13.0	9.0	44.00

Pore Size of Membrane (μm)	Pore Density（well/cm²）
0.4	1x10⁸
1.0	2x10⁷
3.0	2x10⁶
5.0	4x10⁵
8.0	1x10⁵

Cell Culture Inserts, PC Membrane

Individually packaged in peelable Tyvek film and Blister, Sterile

TC Treated Cat. No.	Non-Treated Cat. No.	Product Description	Pore Size (μm)	Membrane Transparence	/Pack	/Case
723001	723011	6 Cell Culture Inserts+6 Well Plate	3.0	Translucent	6	24
724001	724011	12 Cell Culture Inserts+12 Well Plate	3.0	Translucent	12	48
725001	725011	12 Cell Culture Inserts+24 Well Plate	3.0	Translucent	12	48
723101	723111	6 Cell Culture Inserts+6 Well Plate	0.4	Opaque	6	60
724101	724111	12 Cell Culture Inserts+12 Well Plate	0.4	Opaque	12	120
725101	725111	12 Cell Culture Inserts+24 Well Plate	0.4	Opaque	12	120
724201	724211	12 Cell Culture Inserts+12 Well Plate	5.0	Translucent	12	48
725201	725211	12 Cell Culture Inserts+24 Well Plate	5.0	Translucent	12	48
723301	723311	6 Cell Culture Inserts+6 Well Plate	8.0	Translucent	6	24
724301	724311	12 Cell Culture Inserts+12 Well Plate	8.0	Translucent	12	48
725301	725311	12 Cell Culture Inserts+24 Well Plate	8.0	Translucent	12	48
726001	/	100mm Cell Culture Insert-Dish	3.0	Translucent	1	10

Cell Culture Inserts, PET Membrane

Individually packaged in peelable Tyvek film and Blister, Sterile

TC Treated Cat. No.	Non-Treated Cat. No.	Product Description	Pore Size (μm)	Membrane Transparence	/Pack	/Case
723121	723131	6 Cell Culture Inserts+6 Well Plate	0.4	Opaque	6	24
724121	724131	12 Cell Culture Inserts+12 Well Plate	0.4	Opaque	12	48
725121	725131	12 Cell Culture Inserts+24 Well Plate	0.4	Opaque	12	48
723021	723031	6 Cell Culture Inserts+6 Well Plate	3.0	Translucent	6	24
724021	724031	12 Cell Culture Inserts+12 Well Plate	3.0	Translucent	12	48
725021	725031	12 Cell Culture Inserts+24 Well Plate	3.0	Translucent	12	48
723421	723431	6 Cell Culture Inserts+6 Well Plate	1.0	Transparent	6	24
724421	724431	12 Cell Culture Inserts+12 Well Plate	1.0	Transparent	12	48
725421	725431	12 Cell Culture Inserts+24 Well Plate	1.0	Transparent	12	48
723321	723331	6 Cell Culture Inserts+6 Well Plate	8.0	Transparent	6	24
724321	724331	12 Cell Culture Inserts+12 Well Plate	8.0	Transparent	12	48
725321	725331	12 Cell Culture Inserts+24 Well Plate	8.0	Transparent	12	48

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Cell Culture Inserts

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