Annexin V-Biotin Azide-Free Lyophilized Powder

Annexin V-Biotin Azide-Free Lyophilized Powder

Introduction

Elabscience® Annexin V-Biotin Azide-Free Lyophilized Powder is developed for long-term storage and user-defined concentrations of Biotin-conjugated Annexin V. It contains no preservatives like azides or Proclin. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Biotin - conjugated format of this protein, Annexin V- Biotin, binds specifically to the PS displayed on the outer leaflet of apoptotic cell membrane. The signal of Annexin V- Biotin can be collected with flow cytometry or fluorescence microscopy to detect cell apoptosis.

Product Details

Properties

Detection Principle

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner of early apoptotic cells.

Reconstitution

Reconstitute the reagent with sterile deionized water. Make sure the final concentration is less than 1 mg/ml. (This concentration is high enough for stock solution. In our internal tests, working solutions with 1~2 μg/mL Annexin V-Biotin have best results for flow cytometry.) For example, at least 10 μL water should be added to 10 μg Annexin V-Biotin. Otherwise, the lyophilized cake may not be thoroughly soaked in water.

Storage

• The lyophilized powder is stable for at least two years when stored at -20°C in the dark after arrival.
• After reconstitution, the regent can be stored in the dark under sterile conditions at 2~8°C for at least 6 months.

Notes

1. This product is for research use only.
2. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.