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Anti-His(HHHHHH) Affinity Agarose, 2mL– MSE Supplies LLC

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Anti-His(HHHHHH) Affinity Agarose

Anti-His(HHHHHH) Affinity Agarose, 2mL

SKU: EA-IP-008

  • $ 52295



Anti-His(HHHHHH) Affinity Agarose, 2mL

 

Background

Anti-His (HHHHHH) Affinity Agarose, made of high-quality His-tagged antibody covalently conjugated to agarose gel, has the characteristics of high loading capacity, high specificity, stable properties, are reusable, and can be used for immunoprecipitation related experiments such as affinity purification, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP).

 

Performance Index

Scope of application

Affinity purification and immuno(co)precipitation of His-tagged fusion secreted proteins.
His tag can be located at the N-terminus, C-terminus, or middle of the protein, such as the N-terminus His fusion protein (His-Protein), C-terminus His fusion protein (Protein-His), and Met modified intermediate His fusion protein (Met-His-Protein).

Can only be used for affinity purification of secreted proteins.

 

Antibody properties

 

His-Tag Mouse mAb:Mouse IgG.

 

Gel Properties

 

Agarose gel granules, average size 100 μm.

 

Binding capacity

 

1 mL of Sepharose 4B agarose particles, covalently conjugated to 6 mg of mouse-derived IgG.

1 mL affinity gel can purify or precipitate at least 1.2 mg of His fusion protein.

 

 

Liquid storag-

e composition

PBS containing preservatives and 50% glycerol.

 

Storage

-20°C, 12 months.