Anti-V5(GKPIPNPLLGLDST) FAST IP kit(Agarose)

Anti-V5(GKPIPNPLLGLDST)  FAST IP kit(Agarose)

Background

Anti-V5 tag (GKPIPNPLLGLDST) FAST IP kit consists of four components: Anti-V5 Affinity Agarose, Rabbit IgG Affinity Agarose, V5-Tag Mouse mAb and HRP-labeled goat anti-mouse secondary antibody. For rapid, efficient and specific immuno(co)precipitation of V5 tag fusion proteins.

Anti-V5 Affinity Agarose is made by covalently conjugating high-quality V5-Tag Rabbit pAb to agarose gel. It has the characteristics of high loading capacity, high specificity and stable properties; Rabbit IgG Affinity Agarose is used as CoIP experimental control with stable properties; V5-Tag Mouse mAb antibody has the advantages of high specificity, high affinity, and high titer; HRP-labeled goat anti-mouse secondary antibody has been cross-adsorbed and purified, and only recognizes V5-Tag Mouse mAb, without cross reactivity with the heavy and light chains of rabbit polyclonal antibody. The four components have undergone strict quality inspection and can be used individually; the combination of the four components has the advantages of fast, easy and no interference with the strips.

Performance

1.         Scope of application

Immuno(co)precipitation of V5-tagged fusion proteins.

 The V5 tag can be located at the N-terminus, C-terminus or in the middle of the protein, such as N-terminal V5 fusion protein (V5–Protein), C-terminal V5 fusion protein (Protein-V5) and Met-modified N-terminal V5 fusion protein (Met-V5–Protein).

Suitable for secreted proteins.

2.       Antibody properties

V5-Tag Rabbit pAb: rabbit IgG; V5-Tag Mouse mAb: mouse IgG2a subtype.

3.       Gel properties

Agarose gel particles, average particle size 100μm.

4.       Loading Capacity

0.5mL Sepharose 4B agarose particles, covalently conjugated to 4mg Anti-V5 rabbit polyclonal antibody, can purify or precipitate at least 0.6mg V5-labeled protein.

Product Components

Note: Buffer is PBS containing 50% glycerol.

Storage

-20°C, 12 months.