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ATTO WSE-7420 EzRIPA Lysis Kit
SKU: WSE-7420
ATTO WSE-7420 EzRIPA Lysis Kit
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Purpose and Application:
Whole cell extract preparation for the following applications:
- Electrophoresis
- Western blotting
- Immunoprecipitation
- ELISA
- Enzyme activity
- Many more
Features:
- Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
- Standard set includes Protease inhibitor and Phosphatase inhibitor.
- Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.
MSE Supplies is an authorized distributor of ATTO products.
Technical Data:
| SKU# | WSE-7420 |
| ATTO Model | WSE-7420 |
| Applicable | 100 samples, up to 2 x 10⁷ cell/sample |
| Storage | 1 year |
| EzRIPA Lysis buffer |
100 mL (20 mM HEPES pH 7.5, 1% IGEPAL® CA-630*, 0.1% SDS, 0.5% Deoxycholic acid, 150 mM Sodium Chloride) |
| Protease Inhibitor (100x) | 1 mL (Aprotinin, Pepstatin A, Leupeptin) |
| Phosphatase Inhibitor (100X) |
10 mL (Sodium fluoride, Sodium orthovanadate (V), β-sodium glycerophosphate) |
* IGEPAL® CA-630 is the same as NonidetP-40, and a trade mark registration of RhonePoulenc AG Co.