Bioneer AccuPower HotStart PCR PreMix (96 T, 20 μl)

Bioneer AccuPower® HotStart PCR PreMix (96 T, 20 μl)

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AccuPower^® HotStart PCR PreMix enhances response specificity and PCR amplification by applying Bioneer's enzyme-mediated HotStart patent technology. The components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run. In addition, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.

※ This product is shipped at room temperature.

※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

Features and Benefits

Specificity

Effective amplication even with small amounts of template DNA by using Bioneer's differentiated PyroHotstart technology to minimize products from non-specific amplification and maximize efficiency of PCR reaction.

Sensitivity

Excellent sensitivity and amplification efficiency even with small amounts of DNA.

Stability

Enhanced stability allowing delivery at room temperature compared to solution-type products by addition of stabilizing agents.

User-friendly

Shortened optimization time for PCR conditions and accurate acquisition of complicated template DNA with PyroHotstart technology.

Reproducibility

Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables by a mass production under ISO 9001 quality system.

(a) Inhibition of PCR reaction in the zero cycle

After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.

Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.

(b) Denaturation

PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg ions.

The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.

(c) Annealing & Extension

The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.

Applications

• High specificity PCR
• High sensitivity PCR
• gDNA template PCR
• Low - copy target PCR
• Multiple primer pair PCR
• cDNA template PCR

Specifications