Bioneer AccuPower Multiplex PCR PreMix (96 T, 20 μl)
SKU: K-2111
Bioneer AccuPower® Multiplex PCR PreMix (96 T, 20 μl)
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AccuPower® Multiplex PCR PreMix allows to generate 20 multiplexed amplification products in a single tube. By applying antibody-based HotStart Top DNA Polymerase, it reduces non-specific reactions such as mis-priming and primer-dimer during DNA polymerase reaction at a low temperature. This product is applicable for normal and genotyping assays using multiplex PCR technology, along with semi-quantitative gene expression experiments using cDNA.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
Features and Benefits
Multiplex PCR
Generation of 20 multiplexed amplification products in a single tube.
Specificity & Efficiency
Minimized unnecessary reactions such as mis-priming and primer dimer with excellent specificity and amplification efficiency than with other products when compared with same PCR cycles by the use of Hotstart Top DNA polymerase
User-friendly
PCR condition optimization not required with precise specificity of the product only requiring addition of template DNA and primers for anybody to simultaneously amplify the target DNA
Stability
Enhanced stability compared to solution-type products allowing delivery at room temperature by addition of stabilizing agents
Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables through the mass production under ISO 9001 quality system
Applications
- STR analysis
- Molecular diagnostic analysis
- Qualitative, semi-qualitative gene expression assay
- Mutant screening
- Transgenic organism analysis
- Genotyping assay
Specifications
Enzyme | HotStart Top DNA polymerase |
5' to 3' exonuclease | No |
3' to 5' exonuclease | No |
3' – A overhang | Yes |
Fragment Size | Up to 1 kb |