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Bioneer M-MLV Reverse Transcriptase (50,000 Units)– MSE Supplies LLC

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Bioneer M-MLV Reverse Transcriptase (50,000 Units)

Bioneer M-MLV Reverse Transcriptase (50,000 Units)

SKU: E-3122

  • $ 31395



Bioneer M-MLV Reverse Transcriptase (50,000 Units)

Buy Bioneer products from MSE Supplies at the best value.

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase capable of using an RNA molecule as a template to synthesize a double-stranded DNA. It is ideal for use of first-strand synthesis cDNA for Reverse Transcriptase PCR and qRT-PCR

※This product is shipped in dry ice.

 

Features and Benefits

Optimized Buffer Delivery

Guaranteed reliable response by providing optimized for M-MLV reverse transcriptase

Reproducibility

Reproducible results with uniform quality products for each batch under the ISO 9001 quality system.

 

Applications

  • First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR.


Specifications

5' to 3' exonuclease activity No
3' to 5' exonuclease activity No
3' – A overhang No
Strand displacement Yes
Fragment size Up to 9 kb

 

Components

5X reaction buffer: Tris-HCl, KCl, MgCl2(pH 8.1) 100 mM DTT

10 mM dNTPs mix: 2.5 mM of each dNTP

 

Concentration

10,000 Units (200 U/μl)

 

Storage Conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH4)2SO4, Stabilizers.

 

Storage Temperature

-20°C

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A) oligo (dT) as template primer.