Bioneer RocketScript Reverse Transcriptase, RNase H Minus (10,000 Units)

Bioneer RocketScript™ Reverse Transcriptase, RNase H Minus (10,000 Units)

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This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.

※This product is shipped in dry ice.

Features and Benefits

Long cDNA Synthesis

Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H.

Thermostability

Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products.

Sensitivity

Excellent amplification sensitivity even with a trace amount of 1 pg RNA.

Reproducibility

Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system.

Applications

• Gene synthesis
• First-strand synthesis of cDNA from RNA molecules (reverse transcription)
• Random priming reactions
• Library construction
• Probe labeling
• mRNA 5’-end mapping by primer extension analysis
• Real-time PCR
• RT-PCR

Specifications

Components

5X Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.3)

100 mM DTT

10 mM dNTPs mix: 2.5 mM of each dNTP

Concentration

10,000 Units (200 U/μl)

Storage Conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers.

Storage Temperature

-20°C

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmole of deoxynucleotide into acid-precipitable material in 10 min at 37℃ using poly A, oligo dT as template primer.