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Bioneer Taq DNA Polymerase, 10 mM dNTP, 10X Reaction Buffer MgCl2, 20– MSE Supplies LLC

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Bioneer Taq DNA Polymerase, 10 mM dNTP, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

Bioneer Taq DNA Polymerase, 10 mM dNTP, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

SKU: E-2013-1

  • $ 42795

Bioneer Taq DNA Polymerase, 10 mM dNTP, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

Buy Bioneer products from MSE Supplies at the best value.

Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5'-> 3' direction.

Bioneer's Taq DNA Polymerase is isolated from recombinant E.coli strain containing the DNA polymerase gene from Thermus aquaticus YT1. It exhibits its highest activity at pH 9.0 and 72 °C.

 

Features and Benefits

Improved yield & sensitivity: Perform high yield and high sensitivity PCR using Bioneer Taq DNA polymerase.
Versatility: Use for a wide range of DNA amplifications including Real-Time PCR using TaqMan probe
or intercalating dye.
Robust performance: Optimized reaction buffer enhances PCR performance.


Specifications

Concentration: 5 U/ul
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3' -A overhang: Yes
Extension rate: 3-10 kb/min depending on template complexity
Fragment Size: Up to 8 kb from human gDNA and 10 kb from Lambda DNA

 

Application

- Routine PCR
- Intercalating-based qPCR
- Dual-labeled probe based qPCR
- Primer extension
- TA cloning
- Gene sequencing
- Gene expression profiling
- Microbial & viral pathogen detection


Kit Content

Cat. No. Taq DNA Polymerase 10 x Rxn Buffer Dilution Buffer dNTP Mixture MgCl2 Sol.
E-2011 500 units 1 ml (with MgCl2) 1 ml 1 ml -
E-2011-1 500 units 1 ml (without MgCl2) 1 ml 1 ml 1 ml
E-2011-2 500 units 1 ml (with MgCl2) 1 ml - -
E-2011-3 500 units 1 ml (without MgCl2) 1 ml - 1 ml


- 10 x Reaction Buffer without MgCl2: 100 mM Tris-HCl, 400 mM KCl, pH 9.0
- 10 x Reaction Buffer with MgCl2: 100 mM Tris-HCl, 400 mM KCl, 15 mM MgCl2, pH 9.0
- Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 50 % glycerol, pH 8.0
- dNTP Mixture: 10 mM (2.5 mM each dNTP)
- MgCl2 Solution: 20 mM

 

Storage Conditions

Taq DNA Polymerase, including buffers and reagents, should be stored immediately upon receipt at –20°C. If stored in the recommended temperature, this product will be stable until the expiration date printed out on the label.

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.