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$307.95
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Bioneer Top DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)
SKU: E-3101-3
Bioneer Top DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)
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Top DNA polymerase contains a thermostable DNA polymerase having improved polymerase activity by using the recombinant DNA technology on a thermophilic polymerase gene.
※This product is shipped in dry ice.
Features and Benefits
Optimized Buffer Delivery
Guaranteed stable response with Top optimized buffer for DNA polymerase
Efficiency & Sensitivity
High efficiency and sensitivity for DNA amplification using PCR
Reproducibility
Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system
Applications
- Real-time quantification of DNA and cDNA targets using dsDNA binding dye
- Gene expression profiling
- Microbial & viral pathogen detection.
Specifications
| 5' to 3' exonuclease activity | No |
| 3' to 5' exonuclease activity | No |
| 3' – A overhang | Yes |
| Fragment size | Up to 10 kb |
Components
10X reaction buffer with (or without) MgCl2: Tris (pH 9.0), 15 mM MgCl2, etc
1X dilution buffer: 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0
10 mM dNTPs mix: 2.5 mM of each dNTP
Concentration
500 Units (5 U/μl)
Storage Conditions
50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0.
Storage Temperature
-20°C
Unit Definition
One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.