GST pull-downn Kit（Agarose）

GST pull-downn Kit（Agarose）

Background

The GST Pull-Down experiment is based on the reversible specific binding between glutathione-S-transferase (GST) and glutathione (GSH). The corresponding target protein is isolated and purified by GST fused bait protein. The bait protein fuses with GST, and the GST-bait fusion protein binds to GSH-agarose gel complex. If the target protein interacts with the bait protein in the environment, the "agarose gel-GSH-GST-bait protein-target protein" complex will be formed, and then the target protein interacting with the bait protein can be separated and detected.

This product is made of high-quality reduced glutathione (GSH) and agarose gel by covalent binding. It has the characteristics of high binding capacity of protein, fast and convenient operation, strong specificity and specificity, and can specifically combine with proteins containing GST tags in cells or microbial lysates.

Performance

1.         Scope of application

It is derived from the pull down experiment of cells or microbial lysates containing GST labeled fusion protein.

2.         Binding properties

Using 4% agarose gel as the matrix, through the spacer arm of 12 atoms, it is made by chemical covalently combining reduced glutathione.

3.         Gel properties

Agarose gel particles, average particle size 50 μm.

4.         Composition

1 mL of covalently bound GSH gel was stored in 1 mL of PBS containing preservative and 50% glycerol.

Product Components

Storage

-20°C, 12 months.