HA (YPYDVPDYA)-tagged Protein Purification Kit

HA (YPYDVPDYA)-tagged Protein Purification Kit

Background

HA-Tag (YPYDVPDYA)is commonly used as eukaryotic protein recombinant expression marker. The HA tagged fusion protein purification kit is mainly composed of Anti-HA(YPYDVPDYA) affinity agarose, which is made by covalent coupling of high-quality HA antibody and Sepharose 4B agarose particles. It has the characteristics of high binding capacity of protein, high specificity, stability, and repeatability. It can be used for affinity purification of HA tagged fusion proteins.

Performance

1.         Scope of application

Affinity purification of HA tag fusion protein.

HA tag can be located at the N-terminal, C-terminal or middle of the protein, such as N-terminal HA fusion protein (HA–Protein), C-terminal HA fusion protein (Protein–HA) and Met modified N-terminal HA fusion Protein (Met–HA–Protein).

2.         Gel properties

Agarose gel granules, average size 50 μm.

3.         Binding capacity

1mL Sepharose 4B agarose granules, covalently coupled with 6mg Anti-HA monoclonal antibody.

1mL affinity gel can purify at least 1.2mg HA fusion protein.

4.         Repeatability

It can be used repeatedly for more than 5 times.

5.         Components

1mL Anti-HA affinity gel, stored in 1mL PBS containing preservatives and 50% glycerol.

Product Components

Storage

-20°C, 12 months.