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High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)
SKU: E-BC-K221-96
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High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)
| SKU # | E-BC-K221 |
| Detection Instrument | Microplate reader (546 nm), Biochemistry analyzer (546 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | HDL-C |
| Sample Type | Serum, plasma, cells, culture supernatant, tissue |
| Detection Range | 0.065-3.8 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
B A S Messiha et al found that perindopril ameliorates Alzheimer’s disease progression. High density lipoprotein cholesterol (HDL-C) levels in rat serum and hippocampus were determined using HDL-C colorimetric assay kit (E-BC-K221).
TC levels in serum was significantly decreased in OVX/HFFD/D-Gal group, and increased in perindopril treatment group (a. significant change with normal control; b. significant change with OVX/HFFD/D-Gal). No significant difference of HDL-C level can be observed in hippocampal tissue.
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
The generated red purple pigment have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the HDL-C content in the sample can be calculated.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Enzyme Working Solution 1 | 18 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 2 | Enzyme Working Solution 2 | 6 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 3 | Standard (Refer to the label for concentration) | Powder × 1 vial | 2-8℃, 12 months shading light |
| Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.54 | 1.60 | 2.70 |
| %CV | 3.3 | 3.0 | 2.7 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.54 | 1.60 | 2.70 |
| %CV | 4.7 | 5.6 | 4.7 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 95%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (mmol/L) | 0.85 | 2.4 | 3.3 |
| Observed Conc. (mmol/L) | 0.8 | 2.2 | 3.1 |
| Recovery rate (%) | 99 | 92 | 94 |
Sensitivity
The analytical sensitivity of the assay is 0.06 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.