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Hydroxyl Free Radical Scavenging Capacity Assay Kit
SKU: E-BC-K527-M-96
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Hydroxyl Free Radical Scavenging Capacity Assay Kit
| SKU # | E-BC-K527-M |
| Detection Instrument | Microplate reader (500 nm-530 nm, optimum wavelength: 510 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Sample type | Serum, plasma, animal tissue |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 40 min |
| Precision | Average inter-assay CV: 4.600% | Average intra-assay CV: 4.100% |
| Other instruments required | Micropipettor, Vortex mixer, Centrifuge |
| Other reagents required | Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the scavenging capacity detection range (10-50%). The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Dog serum | 1 |
| Rat serum | 1 |
| Cynomolgus monkey serum | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
Note: The diluent is double distilled water.
Detection Principle
Hydroxyl radical is a kind of reactive oxygen species, which can kill red blood cells, degrade DNA, cell membrane and polysaccharide compounds, causing damage to cell structure and function, and then leading to metabolic disorders in the body to cause diseases. The scavenging ability of hydroxyl free radical is one of the important indexes of the antioxidant ability of samples. It has been widely used in the research of antioxidant health care products and drugs.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Substrate A | Powder × 1 vial | Powder × 2 vials | 2-8℃, 12 months shading light |
| Reagent 2 | Substrate B | Powder × 1 vial | Powder × 2 vials | 2-8℃, 12 months shading light |
| Reagent 3 | Substrate C | 12 mL × 1 vial | 24 mL × 1 vial | 2-8℃, 12 months shading light |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Standard Curve
Dilute human serum with double distilled water to different concentrations, then
detect the hydroxyl free radical scavenging capacity, plot the standard curve and
data are provided as below for reference only: