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M199 Complete Medium (20)– MSE Supplies LLC
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M199 Complete Medium (20)

M199 Complete Medium (20)

SKU: PM150610C-500-5

  • $ 99395



M199 Complete Medium (20) 


General Information

Product Introduction M199 (Medium 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic medium, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in COH2 environment, hank's salt is used in non-CO2 environment.
Ingredient Statement

With: M199
Nutrients
1%(V/V)Penicillin-Streptomycin
2mM L-Alanyl-L-Glutamine

Concentration Ready-to-use
Format Liquid
Bacterial Detection Negative
Fungal Detection Negative
Mycoplasmal Detection Negative
Endotoxin Level <3 EU/mL
Storage This product can be stored at 2-8℃ for 3 months with shading light.
Shipping Ice bag
Expiration Date 3 months


Notes

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.
4. It is not suitable for long time storage at room temperature.
5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.