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Malondialdehyde (MDA) Colorimetric Assay Kit (Plant Samples)
SKU: E-BC-K027-M-96
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Malondialdehyde (MDA) Colorimetric Assay Kit (Plant Samples)
| SKU # | E-BC-K027-M |
| Detection Instrument | Microplate reader(525-535 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | MDA |
| Sample Type | Plant tissue |
| Sensitivity | 0.17 nmol/mL |
| Detection Range | 0.17-50 nmol/mL |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 5.100% | Average intra-assay CV: 4.500% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Clarificant | 1.5 mL × 1 vial | 1.5 mL × 2 vials | 2-8℃, 12 months |
| Reagent 2 | Acid Reagent | 45 mL × 1 vial | 45 mL × 2 vials | 2-8℃, 12 months |
| Reagent 3 | Chromogenic Agent | 15 mL × 1 vial | 30 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 4 | 200 nmol/mL Standard | 5 mL × 1 vial | 5 mL × 1 vial | 2-8℃, 12 months |
| Reagent 5 | 10 × Concentrated Extracting Solution | 40 mL × 1 vial | 40 mL × 1 vial | 2-8℃, 12 months |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three carrot samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (nmol/mL) | 0.50 | 15.50 | 35.60 |
| %CV | 4.9 | 4.5 | 4.4 |
Inter-assay Precision
Three carrot samples were assayed 17 times in duplicate by three operators to determine precision between assays
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (nmol/mL) | 0.50 | 15.50 | 35.60 |
| %CV | 6.2 | 6.7 | 6.3 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 100%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (nmol/mL) | 8.5 | 18.3 | 33.5 |
| Observed Conc. (nmol/mL) | 8.6 | 18.1 | 33.5 |
| Recovery rate (%) | 101 | 99 | 100 |
Sensitivity
The analytical sensitivity of the assay is 0.38 nmol/mL MDA. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve:
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (nmol/mL) | 0 | 5 | 10 | 10 | 20 | 30 | 40 | 50 |
| Average OD | 0.040 | 0.138 | 0.242 | 0.333 | 0.442 | 0.665 | 0.878 | 1.014 |
| Absoluted OD | 0 | 0.098 | 0.202 | 0.293 | 0.402 | 0.625 | 0.838 | 0.974 |