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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
SKU: E-BC-K025-S-100
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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
| SKU # | E-BC-K025-S |
| Detection Instrument | Spectrophotometer(532 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | MDA |
| Sample Type | Serum, plasma, animal tissue |
| Sensitivity | 0.38 nmol/mL |
| Detection Range | 0.38-133.33 nmol/mL |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 90 min |
| Precision | Average inter-assay CV: 8% | Average intra-assay CV: 4.900% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Human plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Detection Principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Kit Components & Storage
| Item | Component | Size 1 (48 T) | Size 2 (96 T) | Storage |
| Reagent 1 | Clarificant | 12 mL × 1 vial | 24 mL × 1 vial | 2-8℃, 12 months |
| Reagent 2 | Acid Reagent | 12 mL × 1 vial | 12 mL × 1 vial | 2-8℃, 12 months |
| Reagent 3 | Chromogenic Agent | Powder × 1 vial | Powder × 2 vials | 2-8℃, 12 months shading light |
| Reagent 4 | 10 nmol/mL Standard | 5 mL × 1 vial | 5 mL ×1 vial | 2-8℃, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (nmol/mL) | 1.20 | 35.60 | 102.50 |
| %CV | 5.3 | 4.8 | 4.6 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (nmol/mL) | 1.20 | 35.60 | 102.50 |
| %CV | 7.8 | 8.2 | 8.0 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (nmol/mL) | 27.5 | 88.4 | 112.3 |
| Observed Conc. (nmol/mL) | 27.2 | 90.2 | 114.5 |
| Recovery rate (%) | 99 | 102 | 102 |
Sensitivity
The analytical sensitivity of the assay is 0.38 nmol/mL MDA. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.