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Monoamine Oxidase (MAO) Activity Assay Kit
SKU: E-BC-K008-S-100
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Monoamine Oxidase (MAO) Activity Assay Kit
| SKU # | E-BC-K008-S |
| Detection Instrument | Spectrophotometer(355 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | MAO |
| Sample Type | Animal tissue |
| Sensitivity | 6 U/L |
| Detection Range | 6-722 U/L |
| Detection Method | Colorimetric method |
| Assay Time | 80 min |
| Precision | Inter-assay CV: 3.3% | intra-assay CV: 2.7% |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Valid period | 12 months |
Dilution Of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| Human serum | 1 |
The diluent is buffer working solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
MAO can catalysis 4-dimethylambenzylamine to produce p-dimethylaminobenzaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.
Kit Components & Storage
| Item | Component |
Size 1 (50Assays) |
Size 2 (100Assays) |
Storage |
| Reagent 1 | Extraction Solution A | 30 mL ×1 vial | 60 mL ×1 vial | 2-8°C, 12 months |
| Reagent 2 | Extraction Solution B | 60 mL ×1 vial | 60 mL × 2 vials | 2-8°C, 12 months |
| Reagent 3 | Buffer Solution | 60 mL ×1 vial | 60 mL × 2 vials | 2-8°C, 12 months |
| Reagent 4 | Chromogenic Agent | 10 mL ×1 vial | 15 mL ×1 vial | 2-8°C, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
80.00 | 265.00 | 420.00 |
| %CV | 3.2 | 2.4 | 2.5 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
80.00 | 265.00 | 420.00 |
| %CV | 3.5 | 3.0 | 3.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (U/L) | 130 | 325 | 510 |
| Observed Conc. (U/L) | 130.0 | 331.5 | 515.1 |
| Recovery rate (%) | 100 | 102 | 101 |
Sensitivity
The analytical sensitivity of the assay is 6 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.