MSE PRO Albumin, Nuclease-Free

MSE PRO™ Albumin, Nuclease-Free

The MSE PRO™ Bovine Serum Albumin, also known as Bovine Albumin or BSA, is a globulin in bovine serum. It is a 587 amino acid protein composed of three homologous alpha domains. Bovine serum albumin contains 17 disulfide bonds and an unpaired cysteine (Cys34), which promotes dimerization and also affects higher level of aggregation because the rate of aggregation is slowed down if Cys34 is covalently bound to another compound.BSA has a very wide range of laboratory applications, and is often used as a sequestering agent in immunological assays, where it can be treated to remove nucleic acid ectonuclease, endonuclease, ribonuclease, and protease activity. and protease activity. Albumin may undergo some degradation during treatment. It is generally used as a stabilizer in preservation solutions and reaction solutions for restriction or modification enzymes, and also prevents loss of enzymes by adsorption to the tube wall, and can be used as a ballast protein in precipitates of contaminating nucleases and proteases. It can be used as a ballast protein in the precipitation of contaminated nucleases and proteases. It can help the stability and activity of Taq enzyme in PCR system and can improve the efficiency of PCR.

Brand: MSE PRO™ 

Manufacturer: MSE Supplies LLC

MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.

Applications:

BSA is often used as a reaction stabilizer for some enzymes in restriction endonuclease digestion reactions and to prevent it from adhering to the walls of the tubes and the tips of the lances. BSA is also commonly used in the production of biopharmaceuticals or as a nutrient for cell and microbial cultures. Moreover, in serum-free cultures of animal cells, the addition of albumin provides physiological and mechanical protection and carriers. In PCR system, it helps the stability and activity of Taq enzyme, and can improve the efficiency of PCR. It can be used in all kinds of immunoassays, enzyme stabilizers, containment solution in WB, protein standards for protein quantification, and negative control in enzyme activity assay reactions.