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MSE PRO Deoxyribonuclease I, Recombinant
SKU: BO0315
MSE PRO™ Deoxyribonuclease I, Recombinant
The MSE PRO™ Bovine pancreatic deoxyribonuclease I was produced recombinantly in the yeast Pichia pastoris to reduce levels of contaminating RNase and to eliminate potential pathogens associated with animal material. Recombinant production of DNase I in organisms with much lower levels of endogenous RNase greatly facilitates purification of enzymes with undetectable levels of RNase. Recombinant DNase I is suitable for: ^ Removal of genomic DNA from RNA preparations prior to RT-PCR ^ Degradation of DNA templates after transcription reactions ^ Removal of unwanted DNA from samples prior to Northern blotting ^ Removal of DNA during biopharmaceutical and bioprocessing.
Brand: MSE PRO™
Manufacturer: MSE Supplies LLC
MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.
Specifications:
| SKU# | BO0315 |
| Note | Kunitz units reported by other vendors may be 2 to 4 times higher than the Kunitz units measured in our laboratory. As determined in our laboratory, one Kunitz unit digests 1 mg of calf thymus (or pUC19 orl-phage) DNA in 10 minutes in 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8, at 37oC. The correlation between digested units and Kunitz units may be different in other buffer systems. |
| Source | Pichia pastoris |
| Description | Recombinant bovine pancreatic deoxyribonuclease 1 was cultured from Pichia pastoris. Purified by chromatography. Free of animal sources, RNases and proteases. Lyophilized powder containing glycine as stabilizer. |
| Activity | ≥5000 units per mg protein |
| Storage | 2-8°C. Avoid moisture. |
| Unit Definition | When acting on highly polymerized DNA at pH 5.0, one unit at 250°C at 260 nm results in a 0.001 increase in absorbance per minute, the same as our other DNase I products. |
| Note | Product packaging is subject to change, the picture is for reference only |