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MSE PRO Deoxyribonuclease I, Recombinant,AOF Bioprocess Grade
SKU: BO0310
MSE PRO™ Deoxyribonuclease I, Recombinant,AOF Bioprocess Grade
The MSE PRO™ Bovine pancreatic deoxyribonuclease I was produced recombinantly in the yeast Pichia pastoris to reduce levels of contaminating RNase and to eliminate potential pathogens associated with animal material. Recombinant production of DNase I in organisms with much lower levels of endogenous RNase greatly facilitates purification of enzymes with undetectable levels of RNase. Recombinant DNase I is suitable for: ^ Removal of genomic DNA from RNA preparations prior to RT-PCR ^ Degradation of DNA templates after transcription reactions ^ Removal of unwanted DNA from samples prior to Northern blotting ^ Removal of DNA during biopharmaceutical and bioprocessing.
Brand: MSE PRO™
Manufacturer: MSE Supplies LLC
MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.
Specifications:
| SKU# | BO0310 |
| Note | Kunitz units reported by other vendors may be 2 to 4 times higher than the Kunitz units measured by our lab. Based on our laboratory's measurements, one Kunitz Unit can digest 1 mg of calf thymus (or pUC19 or phage) DNA in 10 minutes at 37oC, 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8. The correlation between the digested units and the Kunitz Units may be different in other buffer systems |
| Source | Pichia pastoris |
| Description | Chromatographically purified AOF bioprocessing grade. Contains 2,000 units per lyophilized powder with glycine mg dry weight as stabilizer. Used for DNA removal in bioprocessing and primary stem cell isolation applications. may contain protease and RNase |
| Activity | ≥2,000 Kunitz Units/mg dry wt |
| Storage | 2-8°C. Avoid moisture. Avoid moisture |
| Unit Definition | When acting on highly polymerized DNA at pH 5.0, 1 unit at 250°C, 260 nm results in a 0.001 increase in absorbance per minute, as with our other DNase I products. |
| Note | Product packaging is subject to change, the picture is for reference only |