MSE PRO Ribonuclease A, DNase & Protease Free
SKU: BO0077
MSE PRO™ Ribonuclease A, DNase & Protease Free
The MSE PRO™ Ribonuclease is inhibited by heavy metal ions and competitively inhibited by DNA. Since molecular biology grade RNase A is essentially free of DNase and protease activity, it is suitable for the removal of RNA from DNA in nucleic acid workups, as well as when other enzymes are used or intact proteins must be recovered.
Brand: MSE PRO™
Manufacturer: MSE Supplies LLC
MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.
Specifications:
SKU# | BO0077 |
Stability | Stable at least 2 years at 2-8°C or -20°C |
Technical Notes | Because of the enzyme's affinity for glass surfaces, special care should be taken when handling. In lyophilization and at solution temperatures ≥2°C and low ionic strength, the enzyme remains active but clumps together. Heating the RNase A solution to inactivate the DNase may not be satisfactory because RNase activity may be lost if a precipitate forms and heat-treated DNase may reactivate over time. This product is suitable for applications requiring minimal DNase and protease content without further treatment. |
Source | Source |
Description | Molecular biology grade. Contains approximately 5 mg/ml of 50% glycerol solution. Specifically designed for purification of DNA plasmids. Each lot is tested for DNase and protease. |
Storage | Store at 2-8°C or -20°C |
Activity | ≥2,000 units per mg protein |
Unit Definition | When yeast ribosomal RNA is hydrolyzed to acid-soluble azo-nucleotides at 37°C and pH 5.0, one Karnitzky unit increases the absorbance at 260 nm by 1.0 One Karnitzky unit is equal to 50 of our units. |
Note | Product packaging is subject to change, the picture is for reference only. |