MYC (EQKLISEEDL)-tagged Protein Purification Kit

MYC (EQKLISEEDL)-tagged Protein Purification Kit

Background

MYC-Tag (EQKLISEEDL) is commonly used as eukaryotic protein recombinant expression marker. The MYC tagged fusion protein purification kit is mainly composed of Anti-MYC (EQKLISEEDL) affinity agarose, which is made by covalent coupling of high-quality MYC antibody and Sepharose 4B agarose granules. It has the characteristics of high binding capacity of protein, high specificity, stability, and repeatability. It can be used for affinity purification of MYC tagged fusion proteins.

Performance

1.         Scope of application

Affinity purification of MYC tag fusion protein.

MYC tag can be located at the N-terminal, C-terminal or middle of the protein, such as N-terminal MYC fusion protein (MYC-Protein), C-terminal MYC fusion protein (Protein-MYC) and Met modified N-terminal MYC fusion Protein (Met-MYC-Protein).

2.         Gel properties

Agarose gel granules, average size 50 μm.

3.         Binding capacity

1mL Sepharose 4B agarose granules, covalently coupled with 6mg Anti-MYC monoclonal antibody.

1mL affinity gel can purify at least 1.2mg MYC fusion protein.

4.         Repeatability

It can be used repeatedly for more than 5 times.

5.         Components

1mL Anti-MYC affinity gel, stored in 1mL PBS containing preservatives and 50% glycerol.

Product Components

Storage

-20°C, 12 months.