Total Price: $0.00
Contact Information
Plant Flavonoids Colorimetric Assay Kit
SKU: E-BC-K284-S-500
Plant Flavonoids Colorimetric Assay Kit
| SKU # | E-BC-K284-S |
| Detection Instrument | Spectrophotometer (510 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Sample type | Plant tissue |
| Sensitivity | 0.315 μg/mL |
| Detection range | 0.315-150 μg/mL |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 2.200% | Average intra-assay CV: 1.900% |
| Other instruments required | Vortex mixer, Micropipettor, Air oven , Crusher, Ultrasonic cell processor |
| Other reagents required | 60% Ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.315-150 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Camphor leaves tissue homogenization | 8-15 |
| Green pepper tissue homogenization | 1 |
| Heather | 25-35 |
Note: The diluent is 60% Ethanol.
Detection Principle
In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm.
Kit Components & Storage
| Item | Component |
Size 1 (50 assays) |
Size 2 (100 assays) |
Storage |
| Reagent 1 | 1 mg/mL Standard | 1 mL × 1 vial | 2 mL × 1 vial | 2-8°C, 12 months |
| Reagent 2 | Saline Solution | 2 mL × 1 vial | 4 mL × 1 vial | 2-8°C, 12 months |
| Reagent 3 | Aluminium Reagent | 2 mL × 1 vial | 4 mL × 1 vial | 2-8°C, 12 months |
| Reagent 4 | Alkali Reagent | 25 mL × 1 via | 50 mL × 1 vial | 2-8℃, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μg/mL) | 5.80 | 75.60 | 126.90 |
| %CV | 2.3 | 1.8 | 1.6 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μg/mL) | 5.80 | 75.60 | 126.90 |
| %CV | 1.8 | 2.3 | 2.5 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.
| Parameters | Standard 1 | Standard 2 | Standard 3 |
| Expected Conc. (μg/mL) | 45.5 | 95.5 | 114 |
| Observed Conc. (μg/mL) | 46.0 | 91.7 | 110.6 |
| Recovery rate (%) | 101 | 96 | 97 |
Sensitivity
The analytical sensitivity of the assay is 0.315 μg/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (μg/mL) | 0 | 20 | 60 | 80 | 100 | 120 | 150 |
| Average OD | 0.001 | 0.057 | 0.169 | 0.223 | 0.278 | 0.326 | 0.410 |
| Absoluted OD | 0 | 0.056 | 0.168 | 0.222 | 0.277 | 0.325 | 0.409 |