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Plant Soluble Sugar Colorimetric Assay Kit
SKU: E-BC-K866-M-96
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Plant Soluble Sugar Colorimetric Assay Kit
| SKU # | E-BC-K866-M |
| Detection Instrument | Microplate reader (610-630 nm, optimum wavelength: 620 nm) |
|
Detection method |
Colorimetric method |
Product Details
Properties
| Sample type | Plant sample |
| Detection range | 0.003-1.5 mg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 5.300% | Average intra-assay CV: 1.700% |
| Other instruments required | Centrifuge |
| Other reagents required | Concentrated sulfuric acid, Ethyl acetate |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Mango tissue homogenate | 150-250 |
| 10% Fresh jujube tissue homogenate | 150-250 |
| 10% Grape tissue homogenate | 150-200 |
| 10% Corn tissue homogenate | 100-200 |
| 10% Apple tissue homogenate | 100-150 |
| 10% Banana tissue homogenate | 150-200 |
| 10% Cucumber tissue homogenate | 20-50 |
| 10% Tomato tissue homogenate | 30-60 |
| 10% Mushroom tissue homogenate | 1 |
| 10% Carrot tissue homogenate | 40-60 |
Note: The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor
Detection Principle
Carbohydrate is one of the important components of plant corpus, and is also the main raw material of metabolism and storage material. The kit is used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, quick convenience and suitable for the determination of microscale samples. The detection principle is anthrone colorimetry. Carbohydrate react with anthrone to produce colored material, which has the maximum absorption peak at 620 nm. The soluble sugar content can be determined by measuring the absorbance value.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Substrate | Power × 1 vial | Power × 2 vials | 2-8℃, 12 months, shading light |
| Reagent 2 | 1 mg/mL Standard | 1 mL × 1 vial | 1 mL × 2 vials | 2-8℃, 12 months |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three banana tissue samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mg/mL) | 0.09 | 0.53 | 1.20 |
| %CV | 2.2 | 1.7 | 1.2 |
Inter-assay Precision
Three banana tissue samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mg/mL) | 0.09 | 0.53 | 1.20 |
| %CV | 5.1 | 4.9 | 5.9 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 102%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (mg/mL) | 0.06 | 1.05 | 1.4 |
| Observed Conc. (mg/mL) | 0.1 | 1.1 | 1.4 |
| Recovery rate(%) | 103 | 102 | 101 |
Sensitivity
The analytical sensitivity of the assay is 0.003 mg/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.