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Potassium (K) Turbidimetric Assay Kit
SKU: E-BC-K279-M-96
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Potassium (K) Turbidimetric Assay Kit
| SKU # | E-BC-K279-M |
| Detection Instrument | Microplate reader (450 nm-600 nm, optimum wavelength: 450 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | K |
| Sample type | Serum, plasma, milk, animal tissue, cells, cell culture supernatant |
| Sensitivity | 0.002 mmol/L |
| Detection range | 0.01-0.80 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 6.100% | Average intra-assay CV: 1.100% |
| Other instruments required | Centrifuge, Micropipettor, Vortex mixer |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
B Zeng et al investigate the mechanism of dextran sodium sulfate potentiating NLRP3 inflammasome activation. Potassium (K) level in cell supernatant was determined using potassium turbidimetic assay kit (E-BC-K279-M).
Potassium concentration was significantly changed after treating with DSS and other substances. (*P<0.05, **P<0.01, ***P<0.001)
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.01-0.80 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Rat serum | 1 |
| RAW 264.7 cellular supernatant | 1 |
| Human plasma | 1 |
| Human milk | 1 |
| 10% Rat liver tissue homogenization | 2-4 |
Note: The diluent is deionized water.
Detection Principle
Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm.
Kit Components & Storage
| Item | Component | Size 1 (48 T) | Size 2 (96 T) | Storage |
| Reagent 1 | Precipitant A | 10 mL × 1 vial | 20 mL × 1 vial | 2-8°C, 12 months |
| Reagent 2 | Precipitant B | 1.25 mL × 1 vial | 1.25 mL × 2 vials | 2-8°C, 12 months |
| Reagent 3 | Chromogenic Agent A | 1.25 mL × 1 vial | 12.5 mL × 2 vials | 2-8°C, 12 months |
| Reagent 4 | Chromogenic Agent B | Powder × 1 vial | Powder × 2 vials | 2-8°C, 12 months shading light |
| Reagent 5 | 1 mmol/L Potassium Standard |
1.25 mL × 1 vial | 1.25 mL × 2 vials | 2-8°C, 12 months |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.25 | 0.46 | 0.65 |
| %CV | 1.3 | 1.2 | 0.8 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.25 | 0.46 | 0.65 |
| %CV | 5.8 | 6.4 | 6.1 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 94%.
| Parameters | Standard 1 | Standard 2 | Standard 3 |
| Expected Conc. (mmol/L) | 0.15 | 0.35 | 0.55 |
| Observed Conc. (mmol/L) | 0.1 | 0.3 | 0.5 |
| Recovery rate (%) | 94 | 92 | 96 |
Sensitivity
The analytical sensitivity of the assay is 0.002 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
|
Concentration (mmol/L) |
0 | 0.1 | 0.2 | 0.3 | 0.4 | 0.5 | 0.6 | 0.8 |
| Average OD | 0.044 | 0.108 | 0.203 | 0.275 | 0.356 | 0.429 | 0.507 | 0.654 |
| Absoluted OD | 0 | 0.064 | 0.159 | 0.231 | 0.312 | 0.385 | 0.463 | 0.610 |