QuicKey Human AMH(Anti-Mullerian Hormone) ELISA Kit

SKU: E-TSEL-H0018

$ 331⁹⁵

QuicKey Human AMH(Anti-Mullerian Hormone) ELISA Kit

Detection Range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL

Product Details

Properties

Format	96T
Assay time	2.5h
Sample type	serum and plasma
Sample volume	100μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 0.31-20 ng/mL Sensitivity: 0.19 ng/mL
Application	Quantitative determination of Human AMH concentrations in human serum, plasma and other biological fluids
Reproducibility	Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AMH. You can calculate the concentration of Human AMH in the samples by comparing the OD of the samples to the standard curve.

Kit Components and Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Item	Specifications	Storage conditions after test
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	2-8℃, 1 month
Reference Standard	96T: 2 vials 48T: 1 vial	Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃
Biotinylated Detection Ab Working Solution	1 vial, 6 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	2-8℃ (Protect from light)
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data:

Precision

Intra-assay Precision: 3 samples with low, mid range and high level Human AMH were tested 20 times on one plate, respectively.
Inter-assay Precision: 3 samples with low, mid range and high level Human AMH were tested on 3 different plates, 20 replicates in each plate, respectively.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.93	1.81	8.21	0.99	1.77	7.99
Standard deviation	0.06	0.1	0.43	0.05	0.07	0.3
CV (%)	6.84	5.79	5.26	5.09	4.18	3.71

Recovery

The recovery of Human AMH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	96
EDTA plasma(n=8)	91-103	98

Target Information

Background

AMH is a dimeric glycoprotein exclusively expressed by granulosa cells of pre-antral and small antral follicles in the ovary [1]. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-β (TGF-β) family. It has a molar mass of 140 KDa [2]. In humans, the gene for AMH is AMH, on chromosome 19p13.3. AMH is secreted by sertoli cells of the testes during embryogenesis of the fetal male. In females, it is secreted by the granulosa cells of ovarian follicles. The measurement of AMH in serum has potential clinical utility for determining primary ovarian insufficiency, the success of assisted reproductive therapies, and the detection of ovarian pathologies such as polycystic ovary syndrome [1].
1. van Disseldorp J, Faddy M J, Themmen A P N, et al. Relationship of Serum Antimüllerian Hormone Concentration to Age at Menopause [J]. Obstetrical & Gynecological Survey, 2008, 63(10): 642-643.
2. Hampl R, Snajderova M, Mardesic T. Antimüllerian hormone (AMH) not only a marker for prediction of ovarian reserve [J]. Physiological research, 2011, 60(2): 217.

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results