QuicKey Human PCT(Procalcitonin) ELISA Kit

SKU: E-TSEL-H0002

$ 331⁹⁵

QuicKey Human PCT(Procalcitonin) ELISA Kit

Detection Range	0.31-20 ng/mL
Sensitivity	0.08 ng/mL

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	2.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.08 ng/mL
Sample type &Sample volume	serum, plasma, urine; 50μL
Specificity	This kit recognizes Human PCT in samples. No significant or interference between Human PCT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PCT concentrations in serum, plasma, urine.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Samples (or Standards) and biotinylated detection antibody specific for Human PCT are added to the micro ELISA plate wells. Human PCT would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve.

Kit Components and Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Item	Specifications	Storage conditions after test
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	2-8℃, 1 month
Reference Standard	96T: 2 vials 48T: 1 vial	Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃
Biotinylated Detection Ab Working Solution	1 vial, 6 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	2-8℃ (Protect from light)
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data:

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PCT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PCT were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.99	2.49	9.15	0.97	2.47	8.00
Standard deviation	0.06	0.12	0.40	0.06	0.12	0.39
CV (%)	6.06	4.82	4.37	6.19	4.86	4.88

Recovery

The recovery of Human PCT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-102	99
EDTA plasma(n=8)	86-99	93
Urine(n=8)	87-98	90

Target Information

Background

Procalcitonin (PCT), the precursor of the hormone calcitonin, comprises 116 amino acids and is present in minute quantities under healthy conditions[1]. PCT and other calcitonin precursors in serum are present at less than 50 pg/mL in healthy individuals, but are highly elevated in serum where conditions leading to systemic inflammatory response syndrome or sepsis prevail. The level of PCT has been observed to increase significantly with bacterial infection and correlate to the severity of the infection. Therefore, serum PCT has been considered a powerful biomarker for the diagnosis of bacterial infection[2]. PCT has been proven to have high accuracy as a marker of infection in adult patients, particularly in sepsis. In children it has been investigated in several conditions, including neonatal sepsis, severe infection in neutropenic children, and other specific bacterial infections (e.g. urinary tract infections, pneumonia, bone and joint tissue infections).

1. Christ-Carin M. Procalcitonin in bacterial infections-hype, hope, more or less?[J]. Swiss medical weekly, 2005, 135(3132).

2. Brechot N, Hekimian G, Chastre J, et al. Procalcitonin to guide antibiotic therapy in the ICU[J]. International journal of antimicrobial agents, 2015, 46: S19-S24.

Database Links	SwissProt: P01258 Entrez Gene: 796
Synonyms	PCT
Research Area	Cancer, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results