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Recombinant Human 14-3-3 sigma/YWHAS Protein
SKU: PKSH031400-100
Recombinant Human 14-3-3 sigma/YWHAS Protein
| SKU # | PKSH031400 |
| Expression Host | E.coli |
Description
| Synonyms | 14-3-3 Protein Sigma, Epithelial Cell Marker Protein 1, HME1, SFN, Stratifin |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ser 248 |
| Accession | NP_006133.1 |
| Calculated Molecular Weight | 28.0 kDa |
| Observed Molecular Weight | 28 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, 10mM GSH, 25% glycerol, pH 8.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
14-3-3 protein sigma (YWHAS); also known as stratifin (SFN) and epithelial cell marker protein 1; is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases; phosphatases; and transmembrane receptors; and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2; and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally; YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.