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Recombinant Human APE1/APE Protein
SKU: PKSH032090-50
Recombinant Human APE1/APE Protein
| SKU # | PKSH032090 |
| Expression Host | E.coli |
Description
| Synonyms | AP Endonuclease 1, APE, APE-1REF-1, APE1, APEN, APEX, APEX Nuclease, APEX1, APX, Apurinic-Apyrimidinic Endonuclease 1, DNA-(Apurinic or Apyrimidinic Site) Lyase, HAP1, REF1, Redox Factor-1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Pro2-Leu318 |
| Accession | AAH02338.1 |
| Calculated Molecular Weight | 35.6 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
Background
Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases; APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors; such as c-Jun; c-Fos; NF-kB; p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.