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Recombinant Human ASF1A Protein (His&T7 Tag)
SKU: PKSH032549-50
Recombinant Human ASF1A Protein (His&T7 Tag)
| SKU # | PKSH032549 |
| Expression Host | E.coli |
Description
| Synonyms | ASF1A, Anti-Silencing Function Protein 1 Homolog A, CCG1-Interacting Factor A, CIA, Histone Chaperone ASF1A, hAsf1, hAsf1a, hCIA |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Met204 |
| Accession | Q9Y294 |
| Calculated Molecular Weight | 25.4 kDa |
| Observed Molecular Weight | 22 kDa |
| Tag | N-T7 & C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.