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Recombinant Human ATG10 Protein (His&T7 Tag)
SKU: PKSH032097-50
Recombinant Human ATG10 Protein (His&T7 Tag)
| SKU # | PKSH032097 |
| Expression Host | E.coli |
Description
| Synonyms | APG10-Like, ATG10, Autophagy-Related Protein 10, Ubiquitin-Like-Conjugating Enzyme ATG10 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Thr190 |
| Accession | Q9H0Y0 |
| Calculated Molecular Weight | 24.5 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | N-T7 & C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 20% Glycerol, 0.02% Tween 80, 3mM TCEP, pH 8.5. |
| Reconstitution | Not Applicable |
Background
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.