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Recombinant Human Biotinidase/BTD Protein (His Tag)(Active)
SKU: PKSH030529-50
Recombinant Human Biotinidase/BTD Protein (His Tag)(Active)
| SKU # | PKSH030529 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | BTD |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Asp543 |
| Accession | P43251 |
| Calculated Molecular Weight | 58.2 kDa |
| Observed Molecular Weight | 66-76 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.