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Recombinant Human Brain Natriuretic Peptide/BNP Protein (His & Flag Ta– MSE Supplies LLC

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Recombinant Human Brain Natriuretic Peptide/BNP Protein (His & Flag Tag)

SKU: PKSH032127-50

  • $ 33795



Recombinant Human Brain Natriuretic Peptide/BNP Protein (His & Flag Tag)

 

SKU # PKSH032127
Expression Host E.coli

 

 

Description

Synonyms BNP, Gamma-brain natriuretic peptide, NPPB, Natriuretic peptides B
Species Human
Expression Host E.coli
Sequence His27-Arg102
Accession P16860
Calculated Molecular Weight 11.0 kDa
Observed Molecular Weight 14 kDa
Tag N-His-Flag
Bio-activity Not validated for activity
  

 

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.



Background

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP); a useful marker of heart failure (HF); is considered to be secreted mainly from the ventricle; increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.