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Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)(Active)
SKU: PKSH032156-50
Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)(Active)
| SKU # | PKSH032156 |
| Expression Host | E.coli |
Description
| Synonyms | CA-I, CA1, CAB, Carbonate Dehydratase I, Carbonic Anhydrase 1, Carbonic Anhydrase B, Carbonic Anhydrase I |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Phe261 |
| Accession | P00915 |
| Calculated Molecular Weight | 29.9 kDa |
| Observed Molecular Weight | 25-35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 12.5mM Tris-HCl, 75mM NaCl, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.