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Recombinant Human Carbonic Anhydrase 10/CA10 Protein (Human Cells, His Tag)
SKU: PKSH032157-50
Recombinant Human Carbonic Anhydrase 10/CA10 Protein (Human Cells, His Tag)
| SKU # | PKSH032157 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CA-RP X, CA-RPX, CA10, CARP X, CARPX, Carbonic Anhydrase-Related Protein 10, Carbonic Anhydrase-Related Protein X, Cerebral Protein 15, HUCEP-15 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln22-Asn300 |
| Accession | Q9NS85 |
| Calculated Molecular Weight | 32.8 kDa |
| Observed Molecular Weight | 34 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes; which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration; renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e.; the reversible hydration of CO2) due to point mutations in the zinc binding site; it has esterase activity. The human and mouse CA10 are expressed in the brain; indicating that they may play a role in brain development.