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Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)
SKU: PKSH031567-50
Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)
| SKU # | PKSH031567 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CPB, CPB1, Carboxypeptidase B, PASP, PCPB, Pancreas-Specific Protein |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Tyr 417 |
| Accession | NP_001862.2 |
| Calculated Molecular Weight | 47.0 kDa |
| Observed Molecular Weight | 45 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm. The specific activity is > 10000 pmoles/min/μg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.