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Recombinant Human CD19/Leu-12 Protein (Fc Tag)(Active)
SKU: PKSH032206-50
Recombinant Human CD19/Leu-12 Protein (Fc Tag)(Active)
| SKU # | PKSH032206 |
| Expression Host | CHO Stable Cells |
Description
| Synonyms | B-Lymphocyte Antigen CD19, B-Lymphocyte Surface Antigen B4, CD19, CVID3, Differentiation Antigen CD19, T-Cell Surface Antigen Leu-12 |
| Species | Human |
| Expression Host | CHO Stable Cells |
| Sequence | Pro20-Lys291 |
| Accession | P15391 |
| Calculated Molecular Weight | 57.3 kDa |
| Observed Molecular Weight | 80-95 kDa |
| Tag | C-Fc |
| Bio-activity | Immobilized Human FMC63 at 2μg/ml(100 μl/well) can bind Human CD19-Fc. The ED50 of Human CD19-Fc is 55.28 ug/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact; it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation; the cytoplasmic tail of CD19 becomes phosphorylated; which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency; hypogammaglobulinemia; recurrent bacterial infections and an inability to mount an antibody response to antigen.