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Recombinant Human CD3 ε/CD3E Protein (His Tag)(Active)
SKU: PKSH033388-50
Recombinant Human CD3 ε/CD3E Protein (His Tag)(Active)
| SKU # | PKSH033388 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD3 epsilon, CD3E, CD3e, IMD18, T-Cell Surface Antigen T3/Leu-4 Epsilon Chain, T-Cell Surface Glycoprotein CD3 Epsilon Chain, T3E |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asp23-Asp126 |
| Accession | P07766 |
| Calculated Molecular Weight | 12.8 kDa |
| Observed Molecular Weight | 15-21 kDa |
| Tag | C-His |
| Bio-activity | Immobilized Human CD3E-His at 10 ug/ml (100 ul/wel)can bind Human Anti-CD3 mAb. The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.