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Recombinant Human Collagen α-1(III) Chain/COL3A1 protein (His tag)
SKU: PDEH100164-100
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Recombinant Human Collagen α-1(III) Chain/COL3A1 protein (His tag)
| SKU # | PDEH100164 |
| Expression Host | E. coli |
Description
| Synonyms | Collagen alpha-1(III) chain;CO3A1_HUMAN;CO3A1;PⅢCP |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Asp 1222-Leu 1466 |
| Accession | p02461 |
| Mol_Mass | 26.8 kDa |
| AP_Mol_Mass | 35 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Collagen type III is found in most soft connective tissues, such as skin, lung, and the vascular system, often in association with type I collagen. It is a trimer of alpha 1(III) chains that are linked by interchain disulfide bonds. Defective collagen type III is a cause of Ehlers-Danlos syndrome types III and IV and arterial aneurisms (1). This recombinant mini pro-alpha 1(III) collagen consists of a triple-helical region of Gly-Xaa-Yaa repeats, a short non-helical region, and the C?terminal propeptide. The C?terminal propeptide can be removed by the procollagen C?proteinase (BMP-1).