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Recombinant Human CXCL7/NAP-2 Protein
SKU: PKSH032305-100
Recombinant Human CXCL7/NAP-2 Protein
| SKU # | PKSH032305 |
| Expression Host | E.coli |
Description
| Synonyms | C-X-C Motif Chemokine 7, CTAP3, CXCL7, LDGF, Leukocyte-Derived Growth Factor, MDGFSmall-Inducible Cytokine B7, Macrophage-Derived Growth Factor, PBP, PPBP, Platelet Basic Protein, SCYB7, TGB1, THBGB1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala59-Asp128 |
| Accession | P02775 |
| Calculated Molecular Weight | 8.4 kDa |
| Observed Molecular Weight | 11 kDa |
| Tag | N-His |
| Bio-activity | Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.The ED50 for this effect is <0.5 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Chemokine (C-X-C motif) Ligand 7 (CXCL7); also known as neutrophil activating peptide 2 (NAP-2); is a member of the CXC chemokines containing an ELR domain (Glu-Leu-Arg tripeptide motif). Similar to other ELR domain containing CXC chemokines; such as IL-8 and the GRO proteins; CXCL7 binds CXCR2; chemoattracts and activates neutrophils. CXCL7; Connective Tissue Activating Protein III (CTAPIII) and βthrombogulin (βTG); are proteolytically processed carboxylterminal fragments of platelet basic protein (PBP) which is found in the alphagranules of human platelets. Although CTAPIII; βTG; and PBP represent amino-terminal extended variants of NAP2 and possess the same CXC chemokine domains; these proteins do not exhibit CXCL7/NAP2 activity. CXCL7 induces cell migration through the G-protein-linked receptor CXCR-2.