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Recombinant Human Deoxyribonuclease 1/DNASE1 Protein (His tag)– MSE Supplies LLC

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Recombinant Human Deoxyribonuclease 1/DNASE1 Protein (His tag)

SKU: PDMH100102-100

  • $ 44995



Recombinant Human Deoxyribonuclease 1/DNASE1 Protein (His tag)

 

SKU # PDMH100102
Expression Host HEK293 Cells

 

Description    

Synonyms DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I, Deoxyribonuclease-1, Dornase alfa, EC 3.1.21.1
Species Human
Expression Host HEK293 Cells
Sequence Met 1-Lys282
Accession P24855
Calculated Molecular Weight 30.9 kDa
Observed Molecular Weight 40 kDa
Tag C-His
Bio-activity Not validated for activity
  

 

Properties

Purity > 95% as determined by reducing SDS-PAGE.
Endotoxin Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.


Background

DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.