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Recombinant Human ECE-2 Protein (His Tag)
SKU: PKSH031761-20
Recombinant Human ECE-2 Protein (His Tag)
| SKU # | PKSH031761 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ECE2, KIAA0604, MGC17664, MGC2408, MGC78487, hCG_2022032 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gly 199-Trp 883 |
| Accession | NP_055508.3 |
| Calculated Molecular Weight | 80.2 kDa |
| Observed Molecular Weight | 120-125 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Endothelin-converting enzyme 2; also known as ECE-2; is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET; the biologically inactive precursor of mature ET. Two enzymes; termed ECE-1 and ECE-2; have been molecularly identified. ECE-2 is found primarily in neural tissues; with high levels of expression in midbrain; cerebellum; hypothalamus; frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides; including neurotensin; angiotensin I; substance P; proenkephalin-derived peptides; and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.