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Recombinant Human EGFR/ErbB1 Protein (aa 25-645, Fc Tag)(Active)
SKU: PKSH032398-50
Recombinant Human EGFR/ErbB1 Protein (aa 25-645, Fc Tag)(Active)
| SKU # | PKSH032398 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptortyrosine-protein kinase erbB-1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu25-Ser645 |
| Accession | P00533 |
| Calculated Molecular Weight | 95.7 kDa |
| Observed Molecular Weight | 120-160 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK; PI3 kinase-AKT; PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16; activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling.