Total Price: $0.00
Contact Information
Recombinant Human EGFR/ErbB1 Protein (Fc Tag)(Active)
SKU: PKSH031995-100
Recombinant Human EGFR/ErbB1 Protein (Fc Tag)(Active)
| SKU # | PKSH031995 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ERBB, ERBB1, HER1, NISBD2, PIG61, mENA |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Gly 645 |
| Accession | NP_005219 |
| Calculated Molecular Weight | 95.0 kDa |
| Observed Molecular Weight | 130-140 kDa |
| Tag | C-hFc |
| Bio-activity | Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT). In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.