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Recombinant Human Ephrin-A1/EFNA1 Protein (His Tag)(Active)
SKU: PKSH032388-50
Recombinant Human Ephrin-A1/EFNA1 Protein (His Tag)(Active)
| SKU # | PKSH032388 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | EFNA1, EPH-Related Receptor Tyrosine Kinase Ligand 1, EPLG1, Ephrin-A1, Immediate Early Response Protein B61, LERK-1, LERK1, TNF Alpha-Induced Protein 4, TNFAIP4, Tumor Necrosis Factor Alpha-Induced Protein 4 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asp19-Ser182 |
| Accession | AAH32698.1 |
| Calculated Molecular Weight | 20.4 kDa |
| Observed Molecular Weight | 24-28 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 4% Trehalose, 4% Mannitol, 0.02% Tween 80, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Ephrin-A1 is a member of the A-type ephrin family of cell surface proteins that function as ligands for the A-type Eph receptor tyrosine kinase family. Ephrin-A1 can be induced by TNF and IL1B. Its expression levels can be down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells. Soluble Ephrin-A1 is necessary for the transformation of HeLa and SK-BR3 cells and participates in the relocalization of EPHA2 away from sites of cell-cell contact during transformation. Ephrin-A1 plays an important role in angiogenesis and tumor neovascularization.